GET THE APP

The Impact of Methotrexate on Neural Stem Cells and the Protection Provided by Cells in the Neurogenic Niche | Abstract
Logo

International Journal of Medical Research & Health Sciences (IJMRHS)
ISSN: 2319-5886 Indexed in: ESCI (Thomson Reuters)

Abstract

The Impact of Methotrexate on Neural Stem Cells and the Protection Provided by Cells in the Neurogenic Niche

Author(s):Anmar Alharganee, Haydar Alabedi, Marwah Abdulrahman and Peter Wigmore

While the survival rates for cancer have dramatically increased with the use of chemotherapy, substantial numbers of patients complain of cognitive impairments associated with this treatment. These symptoms are known as “chemobrain” and include loss of concentration and memory associated with poor work performance. One cause of chemobrain appears to be a decrease in the neural stem cell proliferation essential for adult hippocampal neurogenesis. This produces new neurons required for memory consolidation. Neural stem cell proliferation occurs in a stem cell niche within the sub granular zone (SGZ) of the dentate gyrus. Recent in vivo studies have shown that in the SGZ, neural stem cells associated with blood vessels survive chemotherapy better than those not in contact with the vasculature. Our in vitro study tested the effect of Methotrexate (MTX) on dividing neural stem cells and brain endothelial cells separately and when co-cultured in contact with one another. The results, using MTT assays, show that neural cells are significantly more sensitive to MTX than endothelial cells. Marked neural cells were then cultured on a mono layer of either endothelial or non-neural cells (3T3 fibroblasts) and treated with MTX. Co-culture with endothelial cells offered significant protection from MTX compared with culture with non-neural cells or neural cells on their own. This in vitro model replicates in vivo observations and can be used to study the protective role of endothelial cells on neural stem cells. An understanding of this may be used to preserve hippocampal neurogenesis and reduce the incidence of chemobrain. 


Archive
Scope Categories
  • Clinical Research
  • Epidemiology
  • Oncology
  • Biomedicine
  • Dentistry
  • Medical Education
  • Physiotherapy
  • Pulmonology
  • Nephrology
  • Gynaecology
  • Dermatology
  • Dermatoepidemiology
  • Otorhinolaryngology
  • Ophthalmology
  • Sexology
  • Osteology
  • Kinesiology
  • Neuroscience
  • Haematology
  • Psychology
  • Paediatrics
  • Angiology/Vascular Medicine
  • Critical care Medicine
  • Cardiology
  • Endocrinology
  • Gastroenterology
  • Infectious Diseases and Vaccinology
  • Hepatology
  • Geriatric Medicine
  • Bariatrics
  • Pharmacy and Nursing
  • Pharmacognosy and Phytochemistry
  • Radiobiology
  • Pharmacology
  • Toxicology
  • Clinical immunology
  • Clinical and Hospital Pharmacy
  • Cell Biology
  • Genomics and Proteomics
  • Pharmacogenomics
  • Bioinformatics and Biotechnology
  • Google scholar citation report
    Citations : 2979

    International Journal of Medical Research & Health Sciences received 2979 citations as per google scholar report

    International Journal of Medical Research & Health Sciences peer review process verified at publons

    paper.io

    agar io

    wowcappadocia.com
    cappadocia-hotels.com
    balloon-rides.net

    wormax io

    Elektronik Sigara
    Elektronik Sigara