Abstract

The influence of biochanin a consumption on c-CBL-associated protein level in adipose tissue of streptozotocine-nicotinamide induced diabetic rats

Author(s):Ghadimi Darya, Goodarzi Mohammad Taghi, Ziamajidi Nasrin, and Moradkhani Shirin

Increased c-Cbl associated protein (CAP) level in adipose tissue can facilitate glucose uptake via GLUT 4 in adipocytes. Increased level of CAP results from activation of PPARγ transcription factor. Biochanin A, soy isoflavonoid, is a PPARγ agonist. Present study was designed to investigate the influence of Biochanin A supplementation on CAP gene expression and insulin resistance. 12-week-old male Wistar rats were usedin this study.Type 2 diabetes mellitus was induced by intraperitoneal injection of Streptozotocin followed by Nicotinamide. Present study was designed in 8 groups of 5 animals. Biochanin A was supplemented for a month at 4 different doses: 1 and 5 mg/kg; intraperitoneal, 10 and 20 mg/kg; orally. Fasting blood glucose, serum insulin level and HOMA index were examined. CAP level in adipose tissue was tested by immunoblotting analysis. Findings of this study indicated that administration of Biochanin A at doses of 5mg/kg; intraperitoneal, and 20mg/kg; orally, could increase the expression of CAP in adipose tissue significantly. Additionally, insulin resistance improved and fasting blood glucose level decreased considerably. According to the results, increased expression of CAP in adipocytes can be associated with improvement of insulin resistance

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