Women may have persistent risk of HIV acquisition during pregnancy and postpartum . Children acquire infection through mother-to-child transmission (MTCT) either during pregnancy, delivery, or by breast-feeding .evaluating risk of HIV during these periods is important to inform optimal prevention approaches. The aim of this study was to determine the prevalence of TTV and SEN-V among children who borned from HIV-infected mothers by multiplexnested- RT-PCR protocol and ELISA test. Samples were obtained from 100 infants born with HIV- infected mothers. Infants were followed to 18 months of age and plasma was stored within 7 days of birth, at 2, 5, 6, and 8 weeks, and 3, 6, 9, 12 and 18 months. Mothers and their Infants were tested for HIV ,SEN Virus and TTV using multiplexnested- RT-PCR protocol. In this study, Of 100 children ( Female=59, Male=41 ) were born from mothers with HIV who samples were collected after birth. Examined using PCR technique showed of 150 mother who were infected with HIV, 72/150(48%) SENV and 63/150 (42%) TTV . While PCR results showed that infants were infected with HIV,58%TTV positive and 48% SENV positive. In weeks 5 and 10 samples were collected from all infants and were analyzed by PCR technique. There was no difference in the results compared to the seventh day. If babies born with HIV were treated with the ART drugs. There was a high frequency of late diagnosis in this patient cohort. Earlier diagnosis is an important measure for controlling HIV among children and also the results showed that pregnant women with HIV, if, according to a regular schedule ART drugs are prescribed. Reduced risk of transmission to infants.