Aims: The aim is to determine the effect of the hepatitis education provided for nursing students. Methods: The population (n=278) and sample (n=200) of this quasi-experimental study consisted of nursing students. Data were obtained through the “Personal Information Form” and “Hepatitis Knowledge Level Assessment Form” prepared by the researchers. The efficiency of this training was evaluated using the tests that were conducted prior to and posttraining. The analyzing of data was used mean and percentage calculations, The Kruskal-Wallis H, Mann-Whitney U and the Wilcoxon signed-rank tests. Results: It was gathered that there was a statistically significant difference among the total mean pre- and post-test scores in the hepatitis training, and between age, sex, class during the training, status of injection with hepatitis vaccine and the total mean pre-test and post-test scores (p<0.05). There was no significant difference between whether the family or themselves experienced hepatitis and the total mean pre-test and post-test scores (p>0.05). Conclusion: It was determined hepatitis education raised the nursing students’ levels of knowledge and awareness regarding hepatitis. Improving the knowledge levels is not adequate for behavioural change as the rate of vaccinated students is low. Therefore, vaccination campaigns should be organized further in school and country health policies concerning on this matter should be improved accordingly.