The wound infections are among the most common infectious diseases in hospitals. The surgery team members who are in contact with the tissue sections under surgery when doing surgical operations play a significant and substantial role in preventing and controlling the infections through observing the rules and regulations of the infection control in the surgery rooms. The current study has been undertaken for the purpose of determining the surgery room personnel performance regarding the observation of the infection control principles in Jahrom’s training hospital surgery rooms. the current is a descriptive-analytical study of the cross-sectional type which has been conducted in 2011 in Jahrom’s medical sciences university. The total number of 80 individuals from the surgery room personnel participated in the current study. The data collection tool was a checklist which recorded the surgery room personnel’s performance regarding the observation of the infection control in three times in a day through invisible supervision and then according to the scores obtained for each of the personnel their performances were classified in four levels of weak, medium, good and very good. The data gathered through this method was analyzed by SPSS and the statistical tests such as CH-Square tests and Fischer’s exact test. the results of the study indicated that some of the principles of controlling infection in Jahrom’s treatment and training centers surgery rooms are not practiced by the study population and that it has also been found out that the surgery room technicians’ performance is better and the surgeons’ performance is weaker than the others. Besides, the surgery team performance considering the observation of the scrubbing principles is their weakest point in their overall performances. since the results obtained by the author of the current study is indicative of the idea that the study participants are not attentive to some of the infection control principles in the surgery rooms, thus it is suggested that there is a need for in-service training courses, getting the infection control-prevention committees more actively participating and exerting supervisory mechanisms in the surgery rooms and also the use of the new antiseptic materials for surgery room scrubbings, and also it is suggested that the common and traditional scrubbing methods should be replaced by new scrubbing methods.