This study aims to analyze whether the number of trace elements in the Platelet-Rich Fibrin (PRF) substance obtained from rat blood changes with the different tubes used. Trace elements in blood samples were measured using Inductively Coupled Plasma-Mass Spectrometry (ICP-MS) method. Levels of trace elements Aluminum (Al), Titanium (Ti), Zinc (Zn), Copper (Cu), Manganese (Mg), Selenium (Se), Silver (Ag), Nickel (Ni), Cobalt (Co), and Cadmium (Cd) were measured. Trace element levels of Titanium-Platelet-Rich Fibrin (T-PRF) group 2 were higher than the other two groups. Zn, Mg, and Ag levels of this group were significantly different from the other two groups (p<0.05). The group1 had significantly higher Al levels than the sham group and T-PRF had (p<0.05), whereas the Co and Cd levels were not significant. The higher amount of serum trace elements obtained by using titanium tube compared to the other two groups showed that titanium is more biocompatible.