Abstract

Preparation of Bio-Bandage from Human Platelet Lysate Admixed with Sericin Polymer for Efficient Wound Healing

Objectives: A bandage is typically a material that is used to support a dressing, splint, or other medical devices, or it can be used to provide mechanical support to part of the body. The dressing is the component that is applied directly to the wound and is responsible for promoting wound healing. After a skin lesion, the healing process requires the recruitment and activity of different cell types, such as native immune response cells, endothelial progenitor cells, keratinocytes, and fibroblasts.

Materials and methods: Platelet concentrate bag was obtained by apheresis unit (3 days before expiration) from Food and Drug Administration (FDA) regulated blood bank, shipped to the lab at 4°C-8°C temperature using an ice pack, and was placed in storage at -80°C until use. Silk cocoons (Bombyx mori) were collected from the Ram Nagar Silk Cocoon market in well-sterilized plastic bags at ambient temperature and shipped to the lab and stored at room temperature till the cocoons were processed.

Results: The platelet count was (18.5 ± 2.3) × 109 cells/ml. The pH of human Platelet Lysate (hPL) and sericin were checked by digital pH meter and were found 6.8 ± 0.1at 27°C and 6.9 ± 0.1 at 26.5°C respectively. Similarly, hPL and sericin were tested for sterility by the spread plate method. The moisture content of the hPL powder was analyzed by the Karl Fischer method and it was found 1.842% w/w.

Conclusion: Sericin was successfully isolated by the non-chemical method and exhibited good gelling property, became soft and hydrophilic compared to the chemical method, suggesting that sericin may be more suitable for application as a scaffold (wound dressing). Thus our study shows this admixture material is a suitable biological material for many applications in wound management

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